The Incorporation of Porphyrins in Human Leucocytes Measured by Flow Cytometry, Absorption and Emission Spectroscopy.

Alfons Planner*, Rodica-Mariana Ion**, Krzysztof Wiktorowicz*** and Danuta Frackowiak*

* Institute of Physics, Pozna Technical University, Piotrowo 3, 60-965 Pozna , Poland
** ZECASIN, Splaiul Independentei 202, 79611 Bucharest, Rumania
*** Immunodiagnostics Lab., K. Marcinkowski University of Medical Sciences, ul. D uga 1/2 61-848 Pozna, Poland


The incorporation of dye molecules into leukocytes or other cells is important in photodynamic therapy (PDT) applications [1-4]. In clinical medicine, irradiation by visible or near infra red (NIR) light application on various cells is used in diagnostic or therapeutic procedures. PDT is an indirect treatment: the photosensitizers first are applied to the sample and following illumination usually produced singlet oxygen that acts efficiently on the cells.