THERMOPHILIC GLYCOSIDASES, A TOOL FOR THE ENZYMATIC SYNTHESIS OF GLYCOSIDES

Antonio Trincone*

Received 20 December 1996
Accepted 12 January 1997
Published 15 January 1997

Copyright © 1997 Internet Journal of Science - Biological Chemistry

Abstract

Cells and enzymes of thermophilic microorganisms can possess unique features and can offer undoubted advantages under biotechnological and enzymological viewpoints, for applications in the field of biocatalysis. This article is meant to give an overview of principal examples of application of glycosidases from thermophilic organisms, such as Sulfolobus solfataricus, Thermus thermophilus, Pyrococcus furiosus and Caldocellum saccharolyticum, for the synthesis of different glycosides useful in chemical, biological and technological studies.

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Keywords: Glycosidase, enzyme, biocatalysis, carbohydrate, thermophile

Content

• 1. Glycosidases as a tool for the synthesis of glycosidic bond
• 2. Synthesis of glycosides of simple aliphatic and aromatic alcohols and diols
• 3. Stereochemistry of glycosyl transfer: interaction with "glucose" active site of the enzyme
• 4. Stereochemistry of the transfer reaction using simple glucose mimicking molecules
• 5. Target glycosides synthesized by thermophilic glycosidases
• 6. Future prospects
• 7. References
• Acknowledgments

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